Use of multi-primer / multi-gene PCR method for the detection of Mycobacterium tuberculosis among female genital tuberculosis patients in India
نویسندگان
چکیده
Methods A prospective case-control study was undertaken. A total of 302 endo-ovarian tissue biopsies collected from 202 infertile women highly suspected of having genital tuberculosis on laparoscopic examination and from 100 control women of reproductive age. All specimens tested by conventional/phenotypic methods were later compared with multi-gene/ multi-primer PCR method using four sets of primers for detection of Mycobacterium tuberculosis (MTB) in a single tube-single step reaction.
منابع مشابه
Detection of Mycobacterium Tuberculosis among Infertile Patients Suspected with Female Genital Tuberculosis
Female genital tuberculosis (FGTB) is a symptomless disease that evidences itself only when it is investigated for infertility. Demonstration of the etiologic agent by H & E staining or Z-N staining for acid fast bacilli, smear microscopy, culture of menstrual blood, urine and sputum were often unsuccessful. We therefore, proposed to use the endo-ovarian tissue biopsies and pelvic aspirated flu...
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